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- Title
Genetic variation induced by tissue culture in Banana (Musa acuminate L.) cultivar Cavandish Dwarf.
- Authors
Sheidai, Masoud; Aminpoor, Heywa; Noormohammadi, Zahra; Farahani, Farah
- Abstract
An efficient medium culture for clonal mass propagation was established for the propagation of banana (Musa acuminate L.) cultivar Cavendish Dwarf including the salt formulation of Murashige and Skoog, 30 g/L of sucrose, N-phenyl-N-1, 2, 3- thiadiazol 5-yl Urea (0.5 mg/L) and indoleacetic acid (2 mg/L). Fifteen decamer RAPD primers were used to study somaclonal variation among the regenerated plants of the first, third and fifth sub-cultures. Eight out of fifteen primers produced 143 bands in all the genotypes studied. Fifty-three bands (37.70%) were common in the parental genotype as well as in the regenerated plants while 90 bands were polymorph (63.30%). Twenty-five specific bands were observed in the parental genotype and the regenerated plants of different sub-cultures which may be used in genetic descrimination of the genotypes. The presence of specific bands in regenerated plants indicates the rearrangement in certain loci during tissue culture due to somaclonal variation.
- Subjects
BANANAS; PLANT propagation; PLANT tissue culture; RAPD technique; GENOTYPE-environment interaction
- Publication
Gene Conserve, 2010, Vol 9, Issue 35, p1
- ISSN
1808-1878
- Publication type
Article