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- Title
Mitochondrial respiratory chain involvement in peroxiredoxin 3 oxidation by phenethyl isothiocyanate and auranofin
- Authors
Brown, Kristin K.; Cox, Andrew G.; Hampton, Mark B.
- Abstract
Abstract: Mitochondrial peroxiredoxin 3 (Prx 3) is rapidly oxidized in cells exposed to phenethyl isothiocyanate (PEITC) and auranofin (AFN), but the mechanism of oxidation is unclear. Using HL-60 cells deplete of mitochondrial DNA we show that peroxiredoxin 3 oxidation and cytotoxicity requires a functional respiratory chain. Thioredoxin reductase (TrxR) could be inhibited by up to 90% by auranofin without direct oxidation of peroxiredoxin 3. However, inhibition of thioredoxin reductase promoted peroxiredoxin 3 oxidation and cytotoxicity in combination with phenethyl isothiocyanate or antimycin A. We conclude that rapid peroxiredoxin 3 oxidation occurs as a consequence of increased oxidant production from the mitochondrial respiratory chain.
- Subjects
MITOCHONDRIAL membranes; PHYSIOLOGICAL oxidation; AURANOFIN; THIOCYANATES; CELL-mediated cytotoxicity; MITOCHONDRIAL DNA; THIOREDOXIN; OXIDATIVE stress
- Publication
FEBS Letters, 2010, Vol 584, Issue 6, p1257
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2010.02.042