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- Title
Predominantly buried residues in the response regulator Spo0F influence specific sensor kinase recognition
- Authors
McLaughlin, Patrick D.; Bobay, Benjamin G.; Regel, Erin J.; Thompson, Richele J.; Hoch, James A.; Cavanagh, John
- Abstract
Abstract: Several alanine mutations in the response regulator Spo0F induce hypersporulation in Bacillus subtilis. L66A, I90A and H101A mutants are purported to be involved in contacts stabilizing the orientation of the α4-helix and hence the β4–α4 kinase recognition loop. Y13A is thought to affect the orientation of the α1-helix and consequently phosphatase action. Using comparative NMR chemical shift analyses for these mutants, we have confirmed these suppositions and isolated residues in Spo0F critical in sensor kinases discrimination. In addition, we discuss how buried residues and intra-protein communication networks contribute to precise molecular recognition by ensuring that the correct surface is presented.
- Subjects
BACILLUS (Bacteria); ALANINE; MOLECULAR biology; BIOCHEMISTRY
- Publication
FEBS Letters, 2007, Vol 581, Issue 7, p1425
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2007.02.061