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- Title
The erythrocyte skeletons of β-adducin deficient mice have altered levels of tropomyosin, tropomodulin and EcapZ
- Authors
Porro, Fabiola; Costessi, Luisa; Marro, Martín L.; Baralle, Francisco E.; Muro, Andrés F.
- Abstract
The erythrocyte membrane cytoskeleton is organized as a polygonal spectrin network linked to short actin filaments that are capped by adducin at the barbed ends. We have constructed a mouse strain deficient in β-adducin having abnormal erythrocytes. We show here that the levels of several skeletal proteins from β-adducin mutant erythrocytes are altered. In fact, CapZ, the main muscle actin-capping protein of the barbed ends that in the erythrocytes is cytoplasmic, is 9-fold upregulated in mutant skeletons of erythrocytes suggesting a compensatory mechanism. We also detected upregulation of tropomodulin and downregulation of α-tropomyosin and actin. In addition, purified adducin can be re-incorporated into adducin-deficient ghosts.
- Subjects
ERYTHROCYTES; PROTEINS; BLOOD cells; TROPOMYOSINS
- Publication
FEBS Letters, 2004, Vol 576, Issue 1/2, p36
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2004.08.057