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- Title
Effect of citric acid on cell membrane structure and function of Issatchenkia terricola WJL-G4.
- Authors
Meng, Xiangfeng; Liu, Xinyi; Bao, Yihong; Luo, Ting; Wang, Jinling
- Abstract
Aims This study aimed to investigate the changes of cell membrane structure and function of Issatchenkia terricola under citric acid by performing physiological analysis. Methods and results The membrane integrity, surface hydrophobicity, structure, fluidity, apoptosis, and fatty acid methyl esters composition of I. terricola WJL-G4 cells were determined by propidium iodide staining, microbial adhesion to hydrocarbon test, transmission electron microscopy analysis, fluorescence anisotropy, flow cytometry, and gas chromatography-mass, respectively. The results showed that with the increasing of citric acid concentrations, the cell vitality, membrane integrity, and fluidity of I. terricola reduced; meanwhile, apoptosis rate, membrane permeable, hydrophobicity, and ergosterol contents augmented significantly. Compared to control, the activities of Na+, K+-ATPase, and Ca2+, Mg2+-ATPase increased by 3.73-fold and 6.70-fold, respectively, when citric acid concentration increased to 20 g l−1. The cells cracked and their cytoplasm effused when the citric acid concentration reached 80 g l−1. Conclusions I. terricola could successfully adjust its membrane structure and function below 60 g l−1 of citric acid. However, for citric acid concentrations above 80 g l−1, its structure and function were dramatically changed, which might result in reduced functionality.
- Subjects
CITRIC acid; FATTY acid methyl esters; CELL anatomy; FLUORESCENCE anisotropy; MICROBIAL adhesion; TRANSMISSION electron microscopy
- Publication
Journal of Applied Microbiology, 2024, Vol 135, Issue 3, p1
- ISSN
1364-5072
- Publication type
Article
- DOI
10.1093/jambio/lxae057