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- Title
Efficient cloning of tilapia lake virus complementary DNAs using an in vivo strategy in baker's yeast.
- Authors
Cueva, Mario D.; Villena, Gretty K.; Kitazono, Ana A.
- Abstract
Cloning and protein expression in heterologous systems are very useful tools for the study of viral proteins. In this work, an in vivo cloning strategy was applied using the yeast Saccharomyces cerevisiae, as an efficient and low‐cost method to clone several cDNAs from the tilapia lake virus (TiLV). Samples of infected tilapia Oreochromis niloticus tissues were taken and used to isolate their RNA and to obtain and clone the ten viral cDNAs in a shuttle plasmid. The cloning efficiencies range from 5 to 100% but for seven of the cDNAs the values were above 40%, demonstrating the high efficiency of the method.
- Subjects
SACCHAROMYCES cerevisiae; COMPLEMENTARY DNA; PROTEIN expression; DNA viruses; TILAPIA
- Publication
Journal of the World Aquaculture Society, 2021, Vol 52, Issue 6, p1209
- ISSN
0893-8849
- Publication type
Article
- DOI
10.1111/jwas.12776