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- Title
Effects of inhalation of multi-walled carbon nanotube (MWCNT) on respiratory syncytial virus (RSV) infection in mice.
- Authors
Aki Miyauchi; Toshi Akashi; Satoshi Yokota; Yuhji Taquahashi; Akihiko Hirose; Motoki Hojo; Hiroki Yoshida; Masahiko Kurokawa; Wataru Watanabe
- Abstract
Multi-walled carbon nanotubes (MWCNTs), a kind of nanomaterial, are widely used in battery electrodes and composite materials, but the adverse effects associated with their accumulation in the living body have not been sufficiently investigated. MWCNTs are a fibrous material with molecules similar to asbestos fibers, and there are concerns about its effects on the respiratory system. In this study, we conducted a risk assessment by exposing mice using a previously developed nanomaterial inhalation exposure method. We quantified the exposure in the lungs by a lung burden test, evaluated the deterioration due to pneumonia using respiratory syncytial virus (RSV) infection, and measured inflammatory cytokines in bronchoalveolar lavage fluid (BALF). As a result, in the lung burden test, the amount of MWCNT in the lung increased according to the inhalation dose. In the RSV infection experiment, CCL3, CCL5, and TGF-ß, which are indicators of inflammation and lung fibrosis, were elevated in the MWCNTexposed group. Histological examination revealed cells phagocytosing MWCNT fibers. These phagocytic cells were also seen during the recovery period from RSV infection. The present study found that MWCNT remained in the lungs for about a month or more, suggesting that the fibers may continue to exert immunological effects on the respiratory system. Furthermore, the inhalation exposure method enabled the exposure of nanomaterials to the entire lung lobe, allowing a more detailed evaluation of the effects on the respiratory system.
- Subjects
RESPIRATORY syncytial virus; CARBON nanotubes; RESPIRATORY organs; RESPIRATORY syncytial virus infections; MULTIWALLED carbon nanotubes
- Publication
Journal of Toxicological Sciences, 2023, Vol 48, Issue 7, p411
- ISSN
0388-1350
- Publication type
Article
- DOI
10.2131/jts.48.411