We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Comparison of Zn Content in Rapid-acting Insulin and Biphasic Suspension by FAAS.
- Authors
Shkunnikova, Sofia; Širac, Tea; Lalić, Katarina; Kulišić, Ankica; Jablan, Jasna
- Abstract
Various insulin analogs (rapid-acting and intermediate-acting insulin) have been investigated for the determination of zinc content by flame atomic absorption spectrometry. This paper presents the validation of a method and comparison of zinc content in the insulin samples studied. The method was linear (r² = 0.9997), the limit of detection was 0.0098 mg L-1 and the limit of quantification was 0.0296 mg L-1, the precision (as relative standard deviation) was up to 7.4 %, and the accuracy was within a range of 95.6 % to 100.1 % for the recovery of fortified insulin samples. The zinc content in the insulin samples ranged from 14.9 mg L-1 to 16.3 mg L-1 for rapid-acting insulin and 18.7 mg L-1 to 19.9 mg L-1 for intermediate-acting insulin. A higher zinc content was found in the intermediate-acting insulins than in the rapid-acting insulin analogs (p < 0.05). The obtained results should be considered in the establishment of new or improvement of currently available procedures used to assure the quality, safety and efficacy of insulin products.
- Subjects
BIPHASIC insulin; INSULIN derivatives; INSULIN; SOLID phase extraction; DETECTION limit; STANDARD deviations
- Publication
Croatica Chemica Acta, 2021, Vol 94, Issue 2, p95
- ISSN
0011-1643
- Publication type
Article
- DOI
10.5562/cca3830