We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Composition and diversity analysis of the gut bacterial community of the Oriental armyworm, Mythimna separata, determined by culture-independent and culture-dependent techniques.
- Authors
Cai He; Xiaoning Nan; Zhengqing Zhang; Menglou Li
- Abstract
The intestinal bacteria community structure and diversity of the Oriental armyworm, Mythimna separata (Walker) (Lepidoptera: Noctuidae), was studied by analysis of a 16S rDNA clone library, denaturing gradient gel electrophoresis, and culture-dependent techniques. The 16S rDNA clone library revealed a bacterial community diversity comprising Cyanobacteria, Firmicutes, Actinobacteria, Gracilicutes and Proteobacteria, among which Escherichia coli (Migula) (Enterobacteriales: Enterobacteriaceae) was the dominant bacteria. The intestinal bacteria isolated by PCR-denaturing gradient gel electrophoresis were classified to Firmicutes, Proteobacteria, and Gracilicutes, and E. coli was again the dominant bacteria. The culture-dependent technique showed that the intestinal bacteria belonged to Firmicutes and Actinobacteria, and Staphylococcus was the dominant bacteria. The intestinal bacteria of M. separata were widely distributed among the groups Cyanobacteria, Firmicutes, Actinobacteria, Gracilicutes, Proteobacteria, and Gracilicutes. 16S rDNA clone library, denaturing gradient gel electrophoresis, and culturedependent techniques should be integrated to obtain precise results in terms of the microbial community and its diversity.
- Subjects
INSECT diversity; MYTHIMNA separata; GUT microbiome; RECOMBINANT DNA; GEL electrophoresis; MOLECULAR cloning; POLYMERASE chain reaction
- Publication
Journal of Insect Science, 2013, Vol 13, p1
- ISSN
1536-2442
- Publication type
Article
- DOI
10.1673/031.013.16501