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- Title
Association between IGF-1 and IGFBPs in Blood and Follicular Fluid in Dairy Cows Under Field Conditions.
- Authors
Schiffers, Christina; Serbetci, Idil; Mense, Kirsten; Kassens, Ana; Grothmann, Hanna; Sommer, Matthias; Hoeflich, Christine; Hoeflich, Andreas; Bollwein, Heinrich; Schmicke, Marion
- Abstract
Simple Summary: In dairy cows, a hormone called insulin-like growth factor 1 (IGF-1) helps to control growth and reproduction. It affects how oocytes develop in the ovaries. IGF-1 is usually attached to binding proteins (IGFBPs), which can limit its effects. This study looked at how IGF-1 transfers between blood and the fluid around the oocyte. We checked IGF-1 and protein levels in both places and also measured energy levels using ketones. We examined gene activity in ovary cells too to look for local regulation. We found that IGF-1 levels in blood and ovarian fluid are related. High ketone levels, which indicate low energy, were linked to lower IGF-1 and some of the IGFBP. Some gene activity is also related to IGF-1 levels. In summary, while additional research is necessary to fully understand the local regulatory mechanisms, our findings suggest that the transfer of IGF-1 between systems may link metabolic issues to fertility challenges in dairy cows. Insulin-like growth factor 1 (IGF-1) regulates dairy cow reproduction, while the paracrine IGF system locally influences fertility. In both systems, IGF-1 bioactivity is regulated through binding proteins (IGFBPs) inhibiting IGF-1 binding to its receptor (IGF1R). This study aimed to investigate a possible transfer between this endocrine and paracrine system. Therefore, blood and follicular fluid (FF) from postpartum dairy cows were analysed for ß-hydroxybutyrate (BHB), IGF-1, IGFBP-2, -3, -4, -5, and an IGFBP fragment in two study parts. The mRNA expression of IGFBP-2, IGFBP-4, IGF1R, and the pregnancy-associated plasma protein A (PAPP-A) in granulosa cells was measured. The results showed correlations between plasma and FF for IGF-1 (r = 0.57, p < 0.001) and IGFBP-2 (r = −0.57, p < 0.05). Blood BHB negatively correlated with IGF-1 in blood and FF and IGFBP-3, -5 and total IGFBP in blood (IGF-1 plasma: r = −0.26, p < 0.05; FF: r = −0.35, p < 0.05; IGFBP-3: r = −0.64, p = 0.006; IGFBP-5: r = −0.49, p < 0.05; total IGFBP: r = −0.52, p < 0.05). A negative correlation was found between IGFBP-2 expression and IGF-1 concentration in FF (r = −0.97, p = 0.001), while an IGFBP fragment positively correlated with IGF1R-mRNA in FF (r = 0.82, p = 0.042). These findings suggest a transfer and local regulation between the somatotropic axis and the follicular IGF system, linking the metabolic status with local effects on dairy cow fertility.
- Subjects
SOMATOMEDIN C; DAIRY cattle; GRANULOSA cells; BLOOD proteins; CARRIER proteins
- Publication
Animals (2076-2615), 2024, Vol 14, Issue 16, p2370
- ISSN
2076-2615
- Publication type
Article
- DOI
10.3390/ani14162370