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- Title
New dot-blot method for evaluating the effect of inactivators on mite and Japanese cedar pollen allergens.
- Authors
Yoshida, Megumi; Mizukami, Keijiro; Kurata, Keigo; Nasukawa, Tadahiro; Uchiyama, Jumpei; Sakaguchi, Masahiro
- Abstract
As a method of evaluating the effect of inactivators on allergens while suppressing the effect of inactivator on the assay, we developed new dot-blot method that combines immunostaining and protein detection methods. This method is useful for evaluating whether the inactivator can inactivate allergens rather than removing them from the assay.
- Subjects
CRYPTOMERIA japonica; POLLEN; ALLERGENS; MITES; IMMUNOSTAINING
- Publication
Bioscience, Biotechnology & Biochemistry, 2021, Vol 85, Issue 10, p2089
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1093/bbb/zbab146