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- Title
Allyl isothiocyanate suppresses the proteolytic activation of sterol regulatory element-binding proteins and de novo fatty acid and cholesterol synthesis.
- Authors
Miyata, Shingo; Inoue, Jun; Shimizu, Makoto; Sato, Ryuichiro
- Abstract
Sterol regulatory element-binding proteins (SREBPs) are a family of transcription factors that regulate lipid homeostasis by controlling the expression of genes involved in fatty acid and cholesterol synthesis. In this study, we used a stable cell line that expresses a luciferase reporter gene driven by an SRE-containing fatty acid synthase promoter to identify allyl isothiocyanate (AITC), one of the major isothiocyanates in cruciferous vegetables, as a novel SREBP inactivator. We found that AITC downregulated the proteolytic processing of SREBPs and the expression of their target genes in human hepatoma Huh-7 cells. Furthermore, AITC reduced thede novosynthesis of both fatty acids and cholesterol. Our results indicate a novel physiological function of AITC in lipid metabolism regulation. Allyl isothiocyanate suppresses the processing of SREBP-1 and SREBP-2.
- Subjects
ISOTHIOCYANATES; PROTEOLYTIC enzymes; STEROL carrier proteins
- Publication
Bioscience, Biotechnology & Biochemistry, 2016, Vol 80, Issue 5, p1006
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2015.1132154