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- Title
Expression and production of bioactive human interleukin-18 in transgenic tobacco plants.
- Authors
Bin Zhang; Ying-Hua Yang; Yong-Ming Lin; Qing Rao; Guo-Guang Zheng; Ke-Fu Wu
- Abstract
The cDNA of human interleukin-18 (hIL-18) was successfully inserted into the genome of tobacco plant, Nicotiana tabacum cv. NC-89, using Agrobacterium tumefaciens-mediated transformation. Insertion and translation of hIL-18 in transformants were confirmed by PCR, ELISA, and Western blot, respectively. The transformed extracts contained the recombinant hIL-18 protein up to 0.05% of total soluble protein. Activity of the recombinant hIL-18 in plant cells was confirmed by the induction of IFN-γ on IL-18-responsive J6-1 cells by the extracts obtained from the transformants. The expression level of hIL-18 (351 ng g-1 tobacco tissue) obtained in the present study may be sufficient to induce responses/effects in vivo.
- Subjects
INTERLEUKINS; TRANSGENIC plants; TOBACCO; TRANSGENIC organisms; GENOMES; POLYMERASE chain reaction
- Publication
Biotechnology Letters, 2003, Vol 25, Issue 19, p1629
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1025638623770