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- Title
Galactose induces the expression of penicillin acylase under control of the lac promoter in recombinant Escherichia coli.
- Authors
De León, Antonio; Breceda, Graciela B.; Barba de la Rosa, Ana P.; Jiménez-Bremont, Juan F.; López-Revilla, Rubén
- Abstract
The expression of penicillin acylase (PA), cloned in the pPA102 plasmid under control of the wild-type lac promoter and using galactose as inducer in Escherichia coli JM101, JM103 and JM105 transformant cells, was analyzed. The E. coli JM101/pPA102 cultures attained the highest specific activity of PA. For large scale PA production based on E. coli JM101/pPA102 a culture media with galactose instead of isopropyl-thio-galactopyranoside as inducer would be as successful and less expensive.
- Subjects
GALACTOSE; ESCHERICHIA coli; PENICILLIN; BETA lactam antibiotics; ENTEROBACTERIACEAE; GLYCOSIDES
- Publication
Biotechnology Letters, 2003, Vol 25, Issue 17, p1397
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/a:1025035019883