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- Title
Enhanced conversion of sucrose to isomaltulose by a mutant of Erwinia rhapontici.
- Authors
Ahn, Seung-Joon; Yoo, Ji-Hyun; Lee, Hyeon-Cheol; Kim, Sang-Yong; Noh, Bong-Soo; Kim, Jung-Hoe; Lee, Jung-Kul
- Abstract
Mutagenesis of Erwinia rhapontici was performed to enhance the production of isomaltulose from sucrose. A mutant strain, BN 68089, was obtained through a screening process involving automated and miniaturized cultivation in Bioscreen C. This high-throughput, miniaturized screening system was optimized to identify the mutant strain, which had a conversion yield (90%) and productivity (194 g l-1 h-1). The BN 68089 mutant cells were immobilized in sodium alginate and when operated in a packed bed reactor gave a yield of 89% and a productivity of 144 g l-1 h-1 of at 30 °C, the optimal temperature. Immobilized BN 68089 cells exhibited 8% and 15% higher yield and productivity, respectively, than those of the wild-type strain.
- Subjects
SUCROSE; SUGAR; ERWINIA; SACCHARIDES; MUTAGENESIS; BIOTECHNOLOGY
- Publication
Biotechnology Letters, 2003, Vol 25, Issue 14, p1179
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1024554728773