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- Title
Stability of tumor necrosis factor-α during ion exchange chromatography.
- Authors
Ming Li; Yunshan Wang; Weijun Li; Zhiguo Su
- Abstract
Denaturation of target protein by various separation and purification steps contributes significant part to the total product loss in bioseparation. The conformational change and accompanying loss of activity of tumor necrosis factor-α during ion exchange chromatography was reversible and was decreased by adding polyethylene glycol 200 at 2 to 5% (v/v) to the eluting solution.
- Subjects
DENATURATION of proteins; TUMOR necrosis factors; ION exchange chromatography; CHROMATOGRAPHIC analysis; GLYCOLS; POLYETHYLENE glycol
- Publication
Biotechnology Letters, 2003, Vol 25, Issue 11, p905
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1024019817164