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- Title
Proteomic study of the yeast Rhodotorula mucilaginosa RCL-11 under copper stress.
- Authors
Irazusta, Verónica; Estévez, Cristina; Amoroso, María; Figueroa, Lucía
- Abstract
In order to understand the mechanism involved in Rhodotorula mucilaginosa RCL-11 resistance to copper a proteomic study was conducted. Atomic absorption spectroscopy showed that the copper concentration in the medium decreased from 0.5 to 0.19 mM 48 h after inoculation of the yeast. Analysis of one-dimensional gel electrophoresis of crude cell extracts revealed expression of differential bands between cells with and without copper. In order to study this difference, two-dimensional electrophoresis of R. mucilaginosa RCL-11 exposed to Cu for 16, 24, and 48 h was carried out. Identification of differentially expressed proteins was performed by MALDI-TOF/TOF. Ten of the 16 spots identified belonged to heat shock proteins. Superoxide dismutase, methionine synthase and beta-glucosidase were also found over-expressed at high copper concentrations. The results obtained in the present work show that when R. mucilaginosa RCL-11 is exposed to 0.5 mM copper, differential proteins, involved in cell resistance mechanisms, are expressed.
- Subjects
PROTEOMICS; RHODOTORULA mucilaginosa; COPPER; ATOMIC absorption spectroscopy; YEAST
- Publication
BioMetals, 2012, Vol 25, Issue 3, p517
- ISSN
0966-0844
- Publication type
Article
- DOI
10.1007/s10534-012-9531-0