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- Title
Hematological investigations in a case of intravascular hemolysis due to Clostridium perfringens infection.
- Authors
Beljan, Anamarija; Blagec, Viktorija; Bronic, Ana; Pavić, Marina
- Abstract
A patient presented with fever, severe pain and edematous tight due to hip trauma and was scheduled for urgent fasciotomy. Following physical examination, laboratory analyses were requested, and results revealed anemia and severe infection. As the patient's condition was serious, a new set of samples was sent to the laboratory four hours later. Following centrifugation, severely hemolyzed dark-colored serum and plasma samples were obtained and in vitro hemolysis was suspected. The collection of samples was repeated, but a new set of samples was also hemolyzed with a significant decrease in the hemoglobin value. At that point, in vivo hemolysis was suspected, and samples were processed according to standard laboratory procedures for hemolytic samples. Following confirmation of the gas gangrene diagnosis by clinicians, the cause of hemolysis was attributed to the cytotoxic activity of a-toxin produced by the anaerobic gram-positive bacterium Clostridium perfringens. An insight into the laboratory procedure that could help to narrow down the causes of hemolysis and single out C. perfringens as a cause of intravascular hemolysis was given.
- Subjects
CLOSTRIDIUM perfringens; GAS gangrene; HEMOLYSIS &; hemolysins; PAROXYSMAL hemoglobinuria; ANAEROBIC bacteria; GRAM-positive bacteria; CLOSTRIDIUM diseases
- Publication
Biochemia Medica, 2024, Vol 34, Issue 2, p1
- ISSN
1330-0962
- Publication type
Article
- DOI
10.11613/BM.2024.021001