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- Title
Sample type is vital for diagnosing infection with peste des petits ruminants virus by reverse transcription PCR.
- Authors
Luka, Pam Dachung; Ayebazibwe, Chrisostom; Shamaki, David; Mwiine, Frank Norbert; Erume, Joseph
- Abstract
Peste des petits ruminants (PPR) diagnosis from suspected samples from sheep and goats was carried out. Buffy coat, tissues, and oculo-nasal swabs were analyzed using nucleoprotein (NP3/NP4) and fusion protein (F1/F2) gene primers, respectively. Analysis of the sample types and primer set revealed that buffy coat are the best type of samples for PPR diagnosis and the use of two set of primers will increase the number of positives.
- Subjects
PESTE des petits ruminants; SHEEP diseases; GOAT diseases; VETERINARY virology; REVERSE transcriptase polymerase chain reaction; NUCLEOPROTEINS; BUFFY coat; DIAGNOSIS
- Publication
Journal of Veterinary Science, 2012, Vol 13, Issue 3, p323
- ISSN
1229-845X
- Publication type
Article
- DOI
10.4142/jvs.2012.13.3.323