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- Title
T1-mapping for assessment of ischemia-induced acute kidney injury and prediction of chronic kidney disease in mice.
- Authors
Hueper, Katja; Peperhove, Matti; Rong, Song; Gerstenberg, Jessica; Mengel, Michael; Meier, Martin; Gutberlet, Marcel; Tewes, Susanne; Barrmeyer, Amelie; Chen, Rongjun; Haller, Herman; Wacker, Frank; Hartung, Dagmar; Gueler, Faikah
- Abstract
Objectives: To investigate whether T1-mapping allows assessment of acute kidney injury (AKI) and prediction of chronic kidney disease (CKD) in mice. Methods: AKI was induced in C57Bl/6N mice by clamping of the right renal pedicle for 35 min (moderate AKI, n = 26) or 45 min (severe AKI, n = 23). Sham animals served as controls (n = 9). Renal histology was assessed in the acute (day 1 + day 7; d1 + d7) and chronic phase (d28) after AKI. Furthermore, longitudinal MRI-examinations (prior to until d28 after surgery) were performed using a 7-Tesla magnet. T1-maps were calculated from a fat-saturated echoplanar inversion recovery sequence, and mean and relative T1-relaxation times were determined. Results: Renal histology showed severe tubular injury at d1 + d7 in both AKI groups, whereas, at d28, only animals with prolonged 45-min ischemia showed persistent signs of AKI. Following both AKI severities T1-values significantly increased and peaked at d7. T1-times in the contralateral kidney without AKI remained stable. At d7 relative T1-values in the outer stripe of the outer medulla were significantly higher after severe than after moderate AKI (138 ± 2 % vs. 121 ± 3 %, p = 0.001). T1-elevation persisted until d28 only after severe AKI. Already at d7 T1 in the outer stripe of the outer medulla correlated with kidney volume loss indicating CKD (r = 0.83). Conclusion: T1-mapping non-invasively detects AKI severity in mice and predicts further outcome. Key Points: • Renal T1-relaxation times are increased after ischemia-induced acute kidney injury. • Renal T1-values correlate with subsequent kidney volume loss. • T1-mapping detects the severity of acute kidney injury and predicts further outcome.
- Subjects
KIDNEYS; KIDNEY diseases; MAGNETIC resonance imaging; ISCHEMIA; CHRONIC diseases
- Publication
European Radiology, 2014, Vol 24, Issue 9, p2252
- ISSN
0938-7994
- Publication type
Article
- DOI
10.1007/s00330-014-3250-6