We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Target cell induced activation of NK cells in vitro: cytokine production and enhancement of cytotoxic function.
- Authors
Das, Suvendu; Varalakshmi, C.; Kumari, A. Leela; Patel, Megha; Khar, Ashok
- Abstract
This study examines the effect of fixed AK-5 tumour cells on rat NK cells. Co-culture of NK cells with fixed tumour cells augmented the cytotoxicity of NK cells against NK-sensitive targets, YAC-1 and AK-5, and induced the secretion of IFN-γ by NK cells. Antibody against IFN-γ suppressed the anti-tumour activity of NK cells, whereas the addition of T cells during co-culture enhanced this activity. However, macrophages and B cells had no significant effect when present during co-culture with NK cells. All the inducible cytotoxicity was contained within the NK (CD161+) and NKT (CD3+, CD161+) subsets of lymphocytes. However, in the presence of T cells, the cytolytic potential of NKT cells was higher than that of NK cells alone. The augmentation of cytotoxic activity of NK cells by AK-5 cells in presence of T cells was dependent on IL-2 and IFN-γ secretion. NK cell activation was blocked by specific antibodies to IL-2 and IFN-γ in the presence of T cells. Interaction between fixed AK-5 cells with NK and T cell populations induced the expression of Fas-L and perforin in NK cells. These data demonstrate that fixed AK-5 cells initiated cytokine synthesis by NK cells, and the enhanced cytotoxic activity in the presence of T cells was induced as a consequence of the products secreted by activated T lymphocytes. The present observations reflect the possible interactions taking place in vivo after the transplantation of AK-5 tumour in animals. They also suggest direct activation of NK cells after their interaction with the tumour cells.
- Subjects
KILLER cells; LYMPHOCYTES; CANCER cells; LEUCOCYTES; CELL proliferation; B cells
- Publication
Cancer Immunology, Immunotherapy, 2001, Vol 50, Issue 8, p428
- ISSN
0340-7004
- Publication type
Article
- DOI
10.1007/s002620100217