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- Title
Identification and functional characterization of AcerOBP15 from Apis cerana cerana (Hymenoptera: Apidae).
- Authors
Du, Yali; Xu, Kai; Zhao, Huitng; Jiang, Yusuo; Li, Hongquan
- Abstract
Coordination in honeybees is heavily dependent on their chemoreception system, in which odorant-binding proteins are very important. A previous study based on the antennal transcriptome of Apis cerana cerana revealed that OBP15 was significantly up-regulated at 25-day-old. To further unravel and clarify its functional role, we first cloned and characterized AcerOBP15 from the antennae of worker bees. Sequence and phylogenetic analysis showed that AcerOBP15 belongs to the Minus-C OBPs family. AcerOBP15 was primarily expressed in forager antennae and legs. A fluorescent binding assay showed that AcerOBP15 had strong binding affinity to most floral volatiles, a restricted number of bee pheromone components and non-volatile compounds, among which the highest is myrcene. Based on RNAi and EAG assays, AcerOBP15 may be required for myrcene recognition. Taken together, we suggest that AcerOBP15 plays a dual role in olfactory and gustatory reception when foraging. Our study lays a theoretical foundation for further studies regarding the mechanism of chemoreception in A. cerana cerana.
- Subjects
APIS cerana; APIDAE; HYMENOPTERA; BINDING site assay; HONEYBEES; BEES; CHEMICAL senses
- Publication
Apidologie, 2021, Vol 52, Issue 3, p668
- ISSN
0044-8435
- Publication type
Article
- DOI
10.1007/s13592-021-00854-w