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- Title
The identification of the aminoglycoside-phosphorylating enzymes APH(2″) and APH(3′) from the characterization of their reaction products by high performance liquid chromatography.
- Authors
Lovering, A. M.; White, L. O.; Reeves, D. S.
- Abstract
A method is described for the identification of the aminoglycoside-phosphorylating (APH) enzymes APH(2″) and APH(3′) by the high performance liquid chromato-graphic (HPLC) identification of their products of reaction with kanamycin and ATP. Twelve reference strains which produce either APH(2″) or APH(3′) were examined by both this method and the radioenzymatic profile method; correct enzyme identification was made with 12/12 of the strains by the HPLC method and 11/12 of the strains by the radioenzymatic profile method. Reaction products of APH(2″) or APH(3′) with ATP and butirosin, geneticin, lividomycin, ribostamycin, sissomicin or tobramycin were also characterized by HPLC. Conditions for their chromatography are given.
- Publication
Journal of Antimicrobial Chemotherapy (JAC), 1986, Vol 17, Issue 2, p147
- ISSN
0305-7453
- Publication type
Article