We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
First report of raspberry yellows disease caused by raspberry bushy dwarf virus in Japan.
- Authors
Isogai, Masamichi; Yoshida, Mari; Imanishi, Hiroyuki; Yoshikawa, Nobuyuki
- Abstract
Severe yellowing of leaves was observed on red raspberry in Akita Prefecture. When inoculated with sap from symptomatic raspberry leaves, Chenopodium quinoa plants developed chlorotic ringspot and mottling that are typical of raspberry bushy dwarf virus (RBDV) infection. In western blot analysis, an antibody to the coat protein (CP) of RBDV reacted against ca. 30-kDa protein specific to the diseased trees. In RT-PCR testing for RBDV, single DNA fragments were amplified from total RNA samples of the diseased trees. The nucleotide sequences of the DNA fragments covering the entire CP region revealed 87-97 % identities with those of RBDV isolates.
- Subjects
RASPBERRY diseases &; pests; RED raspberry; DNA; WESTERN immunoblotting; COAT proteins (Viruses)
- Publication
Journal of General Plant Pathology, 2012, Vol 78, Issue 5, p360
- ISSN
1345-2630
- Publication type
Article
- DOI
10.1007/s10327-012-0392-8