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- Title
The effect of low-level laser irradiation on hyperglycemia-induced inflammation in human gingival fibroblasts.
- Authors
Lee, Kun-Tsung Denzel; Chiang, Min-Hsuan; Chen, Ping-Ho; Ho, Mei-Ling; Lee, Hong-Zin; Lee, Huey-Er; Wang, Yan-Hsiung
- Abstract
Hyperglycemia-induced inflammation can greatly increase the risk of periodontal disease in people with diabetes. Low-level laser irradiation (LLLI) has been used for wound healing and anti-inflammation in many cases, and LLLI is known to inhibit the lipopolysaccharide (LPS)-stimulated inflammatory response. However, the therapeutic effect of LLLI in diabetes patients with periodontitis remains unknown. In this study, we cultured human gingival fibroblasts (HGFs) in high-glucose medium (35 mM) to mimic a hyperglycemic environment, and then measured the anti-inflammatory effect of LLLI by assessing the expression of pro-inflammatory genes including tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, and IL-8 by quantitative real-time polymerase chain reaction. The results demonstrated no significant inflammatory response in HGFs cultured in mannitol medium and in those treated only with LLLI. However, HGFs cultured only in high-glucose medium showed significantly higher expression of pro-inflammatory cytokine than in those treated together with LLLI. We then observed that LLLI reduced the expression of pro-inflammatory cytokines in HGFs cultured in high-glucose medium by modulating cAMP signaling. We also investigated whether antioxidant (vitamin C) treatment reduced the inflammatory effect of oxidative stress in HGFs cultured in high-glucose medium but found no additive effect upon co-treatment with LLLI, suggesting that LLLI may activate cAMP signaling, but not reactive oxygen species (ROS) signaling, to reduce the high glucose-induced inflammation. In conclusion, LLLI may have an anti-inflammatory effect on HGFs in a high glucose environment and may benefit the treatment of periodontal disease in diabetes patients.
- Subjects
HYPERGLYCEMIA; RISK factors of periodontal disease; MEDICAL lasers; ANTI-inflammatory agents; PEOPLE with diabetes
- Publication
Lasers in Medical Science, 2019, Vol 34, Issue 5, p913
- ISSN
0268-8921
- Publication type
journal article
- DOI
10.1007/s10103-018-2675-6