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- Title
玉米粉液化及糖化工艺条件优化.
- Authors
孙铭泽; 宋遥遥; 卢晓霆
- Abstract
Using corn flour as raw material and dextrose equivalent (DE) value as evaluation index, the effects of different solid-liquid ratio, time, enzyme addition, temperature and pH on the liquefaction and saccharification process of corn flour were optimized. The processing parameters of liquefaction and saccharification were optimized through single factor and orthogonal experiments. Results showed that using corn flour, adding water to prepare slurry with solid-liquid ratio of 1∶4 (g∶ml), adjusting pH to 6.2, the optimal liquefaction conditions were α-amylase addition 8 U/g, liquefaction temperature 80 ℃ and liquefaction time 60 min, and adjusting pH of the liquefied mash to 4.3. The optimal saccharification conditions were glucoamylase addition 250 U/g, saccharification temperature 60 ℃ and time 12 h. Under these conditions, the DE value was 93.1%.
- Subjects
CORN flour; GLUCOAMYLASE; DEXTROSE; RAW materials; SLURRY
- Publication
China Brewing, 2021, Vol 40, Issue 3, p186
- ISSN
0254-5071
- Publication type
Article
- DOI
10.11882/j.issn.0254-5071.2021.03.035