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- Title
啤酒糟生物炭吸附固定化功能菌强化去除多环芳烃特性.
- Authors
许晓毅; 陈小宾; 崔佳豪; 白净; 王斌; 黄天寅; 贺志敏
- Abstract
Biochar prepared at different pyrolysis temperatures from brewer's grains was used as a carrier for the adsorption and immobilization of the PAHs (polycyclic aromatic hydrocarbon) degrading bacteria named SZ-3, whose physicochemical properties and morphology characterization, and their affects on the immobilization of functional bacteria were analyzed. Furthermore, the enhanced microbial degradation efficiency of three species of PAHs (phenanthrene, fluoranthene and pyrene), and their proliferation and enzymatic activity of the functional bacteria during the reaction cycle were investigated. The results indicated that the brewer's grain biochar prepared at a pyrolysis temperature of 500 ℃ was of high hydrophilic property, with a specific surface area and pore size of 2.505 m2 /g and 23.928 nm, respectively; most of the functional groups on the surface of the carbon material were carboxyl groups and fatty enzymes, which had excellent electron transfer properties and possessed a large functional bacteria adsorption capacity of 825.71 nmol P/g; and in the case of the batch experiment, it was showed that the removal rates of phenanthrene, fluoranthene, and pyrene by the optimized SZ-3, which had been prepared from brewer's grain, could attain up to 71%, 38% and 35%, respectively. On the whole, in comparison with the sole biochar and free functional bacteria systems the increase of removal rates of PAHs compounds by use of SZ-3 ranged from 8% to 34%, and particularly, the bio-degradation efficiency of phenanthrene was significantly enhanced. Additionally, the count of functional bacteria increased, and the activity of catechol dioxygenase in the brewer's grain biochar fixed SZ-3 also significantly improved. To conclude, application of brewer's grains biochar as a microbial immobilization carrier is of practical significance in terms of the removal of refractory organic matter.
- Subjects
PHENANTHRENE; POLYCYCLIC aromatic hydrocarbons; CHARGE exchange; FLUORANTHENE; CARBOXYL group; ORGANIC compounds
- Publication
Environmental Science & Technology (10036504), 2022, Vol 45, Issue 12, p9
- ISSN
1003-6504
- Publication type
Article
- DOI
10.19672/j.cnki.1003-6504.1461.22.338