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- Title
Tissue Distribution and Specific Contribution of Arabidopsis FAD7 and FAD8 Plastid Desaturases to the JA- and ABA-Mediated Cold Stress or Defense Responses.
- Authors
Soria-García, Ángel; Rubio, María C; Lagunas, Beatriz; López-Gomollón, Sara; Luján, María de los Ángeles; Díaz-Guerra, Raúl; Picorel, Rafael; Alfonso, Miguel
- Abstract
To overcome the difficulties to analyze membrane desaturases at the protein level, transgenic Arabidopsis plants expressing the plastidial At FAD7 and At FAD8 ω-3 desaturases fused to green fluorescent protein, under the control of their endogenous promoters, were generated and their tissue relative abundance was studied. Gene expression, glucuronidase promoter activity, immunoblot and confocal microscopy analyses indicated that At FAD7 is the major ω-3 desaturase in leaves when compared to At FAD8. This higher abundance of At FAD7 was consistent with its higher promoter activity and could be related with its specificity for the abundant leaf galactolipids. At FAD7 was also present in roots but at much lower level than leaves. At FAD8 expression and protein abundance in leaves was consistent with its lower promoter activity, suggesting that transcriptional control modulates the abundance of both desaturases in leaves. At FAD7 protein levels increased in response to wounding but not to jasmonate (JA), and decreased upon abscisic acid (ABA) treatment. Conversely, At FAD8 protein levels increased upon cold or JA exposure and decreased at high temperatures, but did not respond to ABA or wounding. These results indicated specific and non-redundant roles for the plastidial ω-3 desaturases in defense, temperature stress or phytohormone mediated responses and a tight coordination of their activities between biotic and abiotic stress signaling pathways. Our data suggested that transcriptional regulation was crucial for this coordination. Finally, bimolecular fluorescence complementation analysis showed that both At FAD7 and At FAD8 interact with the At FAD6 ω-6 desaturase in vivo, suggesting that quaternary complexes are involved in trienoic fatty acid production within the plastid membranes.
- Subjects
ABSCISIC acid; JASMONATE; DESATURASES; GREEN fluorescent protein; MEMBRANE proteins; ARABIDOPSIS; FLUORIMETRY
- Publication
Plant & Cell Physiology, 2019, Vol 60, Issue 5, p1025
- ISSN
0032-0781
- Publication type
Article
- DOI
10.1093/pcp/pcz017