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- Title
基于高通量转录组测序筛选马尾松抗松材线虫病相关基因.
- Authors
刘 彬; 刘青华; 周志春; 徐六一; 陈雪莲; 罗 柠
- Abstract
[Objective] To study the genes related to the resistance of Masson pine(Pinus massoniana) to pine wood nematode(Bursaphelenchus xylophilus, PWN). [Method] Based on two genotypes of resistant and susceptible P. massoniana for inoculating pine wood nematode, and sampling at the 1 dpi, 15 dpi and 30 dpi after inoculation, the authors performed a transcriptome analysis to identify differentially expressed constitutive genes associated with resistance to PWN infected. [Result] Comparing resistant and susceptible transcriptomes of P. massoniana inoculated with B. xylophilus, 2 866, 679 and 1 657 differentially expressed genes were obtained at 1, 15, or 30 days post-inoculation(dpi), and there were relatively few common differentially expressed genes at different time points. The GO enrichment analysis was performed on the differentially expressed genes, which indicated that the most significant biological process at the 1 dpi, 15 dpi and 30 dpi was oxidation-reduction process, while the stimulus response, transcriptional regulation and the geranyl diphosphate metabolic process in GO items were also significantly enriched. A further analysis of these related genes in the stimulus response GO term, 26 R genes were found at three time points after inoculation. The expression of the other R genes was constitutive except for two R genes, and no significant difference was observed between PWN and water inoculation. In the transcriptional regulation GO term, only two differentially expressed genes of ERF transcription factors were annotated by nr database, and the rest differentially expressed genes were unknown. After inoculation with PWN, the expression level of ERF transcription factors in resistant P. massoniana was always higher than that in susceptible ones, and there was insignificantly difference between trees inoculating PWN and the control. The result means that expression of the two ERF transcription factors is constitutive. In geranyl diphosphate metabolic process GO term, three GGPPS genes having higher expression level in high resistant P. massoniana, are constitutive expression genes. Here, the authors also found that the TIR-NBS-LRR gene(c65785.graph_c0), ERF transcription factor(c78073.graph_c0), and the three GGPPS genes, having higher expression in resistant P. massoniana, the expression levels were extremely low or even zero in susceptible P. massoniana, which suggested that these genes can be chosen as candidate genes for the development of molecular markers to identify resistant P. massoniana. [Conclusion] Most genes changed the expression level at 1 dpi between resistant and susceptible P. massoniana. The R genes, ERF transcription factors and GGPPS are related to the resistance of P. massoniana, some of which are expected to be developed as molecular markers for identifying PWN-resistance P. massoniana.
- Subjects
PINEWOOD nematode; CONIFER wilt; TRANSCRIPTION factors; TRANSCRIPTOMES; GENOTYPES; VACCINATION
- Publication
Forest Research, 2019, Vol 32, Issue 5, p1
- ISSN
1001-1498
- Publication type
Article
- DOI
10.13275/j.cnki.lykxyj.2019.05.001