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- Title
Synthesis of Cationic Quaternized Nanolevan Derivative for Small Molecule and Nucleic Acid Delivery.
- Authors
Charoenwongphaibun, Chonnipha; Lorthongpanich, Chanchao; Septham, Prapasri; Wangpaiboon, Karan; Panpetch, Pawinee; Pichyangkura, Rath; Charoenwongpaiboon, Thanapon; Kuttiyawong, Kamontip
- Abstract
Levan is a biopolymer composed of fructose chains covalently linked by β−2,6 glycosidic linkages. This polymer self−assembles into a nanoparticle of uniform size, making it useful for a wide range of applications. Also, levan exhibits various biological activities such as antioxidants, anti-inflammatory, and anti-tumor, that make this polymer very attractive for biomedical application. In this study, levan synthesized from Erwinia tasmaniensis was chemically modified by glycidyl trimethylammonium chloride (GTMAC) to produce cationized nanolevan (QA-levan). The structure of the obtained GTMAC−modified levan was determined by FT-IR, 1H-NMR and elemental (CHN) analyzer. The size of the nanoparticle was calculated using the dynamic light scattering method (DLS). The formation of DNA/QA-levan polyplex was then investigated by gel electrophoresis. The modified levan was able to increase the solubility of quercetin and curcumin by 11-folds and 205-folds, respectively, compared to free compounds. Cytotoxicity of levan and QA−levan was also investigated in HEK293 cells. This finding suggests that GTMAC−modified levan should have a potential application for drug and nucleic acid delivery.
- Subjects
CHEMICAL derivatives; SMALL molecules; NUCLEIC acids; BIOPOLYMERS; DRUG delivery systems
- Publication
Gels (2310-2861), 2023, Vol 9, Issue 3, p188
- ISSN
2310-2861
- Publication type
Article
- DOI
10.3390/gels9030188