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- Title
Mutational analysis of theARAFgene in human cancers.
- Authors
Jong Woo Lee; Young Hwa Soung; Su Young Kim; Won Sang Park; Suk Woo Nam; Woo Sung Min; Sang Ho Kim; Jung Young Lee; Nam Jin Yoo; Sug Hyung Lee
- Abstract
Lee JW, Soung YH, Kim SY, Park WS, Nam SW, Min WS, Kim SH, Lee JY, Yoo NJ, Lee SH. Mutational analysis of theARAFgene in human cancers. APMIS 2005;113:54–7.Deregulation of RAS signal transduction has been implicated in the malignant growth of human cancer cells. TheBRAFgene, encoding a RAF family member in the downstream pathway of RAS, is somatically mutated in a number of human cancers, raising the possibility that other RAF family members might be mutated in human cancers. In this study we analyzed the genomic DNAs for the detection of somatic mutations of theARAFgene in 60 human cancer cell lines and 323 primary human cancer tissues, including colorectal carcinomas, gastric carcinomas, ovarian tumors and acute leukemias. The MOLT-4 leukemia cell line was found to harbor anARAFgene mutation resulting in an amino acid substitution (A451T) at the activation segment in the kinase domain of ARAF. In the cancer tissues we could not detect anyARAFgene mutation. Our data indicate that, in contrast to theBRAFgene, theARAFgene is rarely mutated in human cancers, and suggest that alterations of the RAS pathway byARAFgene mutation may not play an important role in the pathogenesis of human cancers.
- Subjects
GENETIC mutation; GENES; CANCER; ONCOGENES; CELL lines
- Publication
APMIS, 2005, Vol 113, Issue 1, p54
- ISSN
0903-4641
- Publication type
Article
- DOI
10.1111/j.1600-0463.2005.apm1130108.x