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- Title
Enhanced Oxygen Availability Improves Liver-specific Functions of the AMC Bioartificial Liver.
- Authors
Poyck, Paul P. C.; Mareels, Guy; Hoekstra, Ruurdtje; van Wijk, Albert C. W. A.; van der Hoeven, Tessa V.; van Gulik, Thomas M.; Verdonck, Pascal R.; Chamuleau, Robert A. F. M.
- Abstract
Long-term culturing of primary porcine hepatocytes (PPH) inside the Academic Medical Center (AMC)-bioartificial liver is characterized by increased anaerobic glycolysis. Recommendations to increase oxygen availability were proposed in a previous numerical study and were experimentally evaluated in this study. Original bioreactors as well as new configuration bioreactors with 2.2-fold thinner nonwoven matrix and 2-fold more capillaries were loaded with PPHs and oxygenated with different gas oxygen pressures resulting in medium pO2 (pO2-med) of either 135-150 mm Hg or 235-250 mm Hg. After 6 days culturing, new configuration bioreactors with pO2-med of 250 mm Hg showed significantly reduced anaerobic glycolysis, 60% higher liver-specific functions, and increased transcript levels of five liver-specific genes compared to the standard bioreactor cultures. Changed bioreactor configuration and increasing pO2-med contributed equally to these improvements. Histological examination demonstrated small differences in cell organization. In conclusion, higher metabolic stability and liver-specific functionality was achieved by enhanced oxygen availability based on a prior modeling concept.
- Subjects
LIVER cells; ACADEMIC medical centers; GLYCOLYSIS; BIOREACTORS; MEDICAL research
- Publication
Artificial Organs, 2008, Vol 32, Issue 2, p116
- ISSN
0160-564X
- Publication type
Article
- DOI
10.1111/j.1525-1594.2007.00500.x