We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Molecular Detection and Associated Risk Factors of Brucella melitensis in Aborted Sheep and Goats in Duhok Province, Iraq.
- Authors
Ameen, Alind M.; Abdulaziz, Nadhim S.; Ghaffar, Nacheervan M.
- Abstract
Brucellosis in sheep and goats has a significant economic and zoonotic impact on the livestock population of Duhok province, Iraq. A total of 681 blood samples from aborted sheep and goats were collected from different flocks in seven districts of Duhok and tested using real-time polymerase chain reaction (RT-PCR). Logistic regression was used for the analysis of the potential risk factors associated with RT-PCR positivity. Results revealed an overall prevalence of 35.45% (CI = 2.57) and 23.8% 18 (CI = 0.44) in sheep and goats, respectively. A statistically significant (p = 0.004) difference in prevalence was found between the two species. RT-PCR detected more positive cases in older-aged animals (OR = 0.7164; p = 0.073). A significant difference was found in RT-PCR positivity in relation to different risk factors, including body condition, treatment taken, and abortion frequency (<0.001). The phylogenetic tree based on the 16S rRNA gene indicated that the isolates belonged to B. melitensis and shared a common ancestor and were genetically related to the United States of America (USA), Greece, China, and Nigeria. This study demonstrates that brucellosis is widely prevalent in the study regions. Therefore, the study suggests the implementation of preventive control measures for brucellosis.
- Subjects
DIHOK (Iraq); GOAT diseases; BRUCELLA melitensis; SHEEP; GOATS; POLYMERASE chain reaction; SHEEP farming; BRUCELLOSIS
- Publication
Pathogens, 2023, Vol 12, Issue 4, p544
- ISSN
2076-0817
- Publication type
Article
- DOI
10.3390/pathogens12040544