We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
In vivo reprogrammed pluripotent stem cells from teratomas share analogous properties with their in vitro counterparts.
- Authors
Choi, Hyun Woo; Kim, Jong Soo; Hong, Yean Ju; Song, Hyuk; Seo, Han Geuk; Do, Jeong Tae
- Abstract
Recently, induced pluripotent stem cells (iPSCs) have been generated in vivo from reprogrammable mice. These in vivo iPSCs display features of totipotency, i.e., they differentiate into the trophoblast lineage, as well as all 3 germ layers. Here, we developed a new reprogrammable mouse model carrying an Oct4-GFP reporter gene to facilitate the detection of reprogrammed pluripotent stem cells. Without doxycycline administration, some of the reprogrammable mice developed aggressively growing teratomas that contained Oct4-GFP+ cells. These teratoma-derived in vivo PSCs were morphologically indistinguishable from ESCs, expressed pluripotency markers, and could differentiate into tissues of all 3 germ layers. However, these in vivo reprogrammed PSCs were more similar to in vitro iPSCs than ESCs and did not contribute to the trophectoderm of the blastocysts after aggregation with 8-cell embryos. Therefore, the ability to differentiate into the trophoblast lineage might not be a unique characteristic of in vivo iPSCs.
- Subjects
PLURIPOTENT stem cells; LABORATORY mice; TROPHOBLAST; STEM cell culture; PROGENITOR cells
- Publication
Scientific Reports, 2015, p13559
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/srep13559