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- Title
A Novel 96well-formatted Micro-gap Plate Enabling Drug Response Profiling on Primary Tumour Samples.
- Authors
Ma, Wei-Yuan; Hsiung, Lo-Chang; Wang, Chen-Ho; Wo, Andrew M.; Chiang, Chi-Ling; Lin, Ching-Hung; Huang, Chiun-Sheng
- Abstract
Drug-based treatments are the most widely used interventions for cancer management. Personalized drug response profiling remains inherently challenging with low cell count harvested from tumour sample. We present a 96well-formatted microfluidic plate with built-in micro-gap that preserves up to 99.2% of cells during multiple assay/wash operation and only 9,000 cells needed for a single reagent test (i.e. 1,000 cells per test spot x 3 selected concentration x triplication), enabling drug screening and compatibility with conventional automated workstations. Results with MCF7 and MDA-MB-231 cell lines showed that no statistical significance was found in dose-response between the device and conventional 96-well plate control. Primary tumour samples from breast cancer patients tested in the device also showed good IC50 prediction. With drug screening of primary cancer cells must consider a wide range of scenarios, e.g. suspended/attached cell types and rare/abundant cell availability, the device enables high throughput screening even for suspended cells with low cell count since the signature microfluidic cell-trapping feature ensures cell preservation in a multiple solution exchange protocol.
- Subjects
CANCER treatment; DOSE-effect relationship in pharmacology; TUMOR dose; MICROPLATES; CELL preservation; CHEMICAL reagents; DRUG utilization
- Publication
Scientific Reports, 2015, p9656
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/srep09656