We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Boosting the Biogenesis and Secretion of Mesenchymal Stem Cell-Derived Exosomes.
- Authors
Wang, Jinli; Bonacquisti, Emily E.; Brown, Adam D.; Nguyen, Juliane
- Abstract
A limitation of using exosomes to their fullest potential is their limited secretion from cells, a major bottleneck to efficient exosome production and application. This is especially true for mesenchymal stem cells (MSCs), which can self-renew but have a limited expansion capacity, undergoing senescence after only a few passages, with exosomes derived from senescent stem cells showing impaired regenerative capacity compared to young cells. Here, we examined the effects of small molecule modulators capable of enhancing exosome secretion from MSCs. The treatment of MSCs with a combination of N-methyldopamine and norepinephrine robustly increased exosome production by three-fold without altering the ability of the MSC exosomes to induce angiogenesis, polarize macrophages to an anti-inflammatory phenotype, or downregulate collagen expression. These small molecule modulators provide a promising means to increase exosome production by MSCs.
- Subjects
EXOSOMES; SECRETION; MESENCHYMAL stem cells; SMALL molecules; STEM cells
- Publication
Cells (2073-4409), 2020, Vol 9, Issue 3, p660
- ISSN
2073-4409
- Publication type
Article
- DOI
10.3390/cells9030660