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- Title
Note: Removal of the Bloom Syndrome DNA Helicase Extends the Utility of Imprecise Transposon Excision for Making Null Mutations in Drosophila.
- Authors
Witsell, Alice; Xane, Daniel P.; Rubin, Sarah; McVey, Mitch
- Abstract
Transposable elements are frequently used in Drosophila melanogaster for imprecise excision screens to delete genes of interest. However, these screens are highly variable in the number and size of deletions that are recovered. Here, we show that conducting excision screens in mus309 mutant flies that lack DmBIm, the Drosophila ortholog of the Bloom syndrome protein, increases the percentage and overall size of flanking deletions recovered after excision of either P or Minos elements.
- Subjects
DNA helicases; DROSOPHILA melanogaster; GENES; GENETIC mutation; MOBILE genetic elements; GENETIC research
- Publication
Genetics, 2009, Vol 183, Issue 3, p1187
- ISSN
0016-6731
- Publication type
Article
- DOI
10.1534/genetics.109.108472