We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Remarkable Site Specificity of Local Transposition Into the Hsp70 Promoter of Drosophila melanogaster.
- Authors
Shilova, Victoria Y.; Garbuz, David G.; Myasyankina, Elena N.; Bing Chen; Evgen'ev, Michael B.; Feder, Martin E.; Zatsepina, Olga G.
- Abstract
Heat-shock genes have numerous features that ought to predispose them to insertional mutagenesis via transposition. To elucidate the evolvability of heat-shock genes via transposition, we have exploited a local transposition technique and Drosophila melanogaster strains with EPgy2 insertions near the Hsp70 gene cluster at 87A7 to produce numerous novel EPgy2 insertions into these Hsp70 genes. More than 50% of 45 independent insertions were made into two adjacent nucleotides in the proximal promoter at positions -96 and -97, and no insertions were into a coding or 3'-flanking sequence. All inserted transposons were in inverse orientation to the starting transposon. The frequent insertion into nucleotides -96 and -97 is consistent with the DNase hypersensitivity, absence of nucleosomes, flanking GAGA-factor-binding Sites, and nucleotide sequence of this region. These experimental insertions recapitulated many of the phenotypes of natural transposition into Hsp70: reduced mRNA expression, less Hsp70 protein, and decreased inducible thermotolerance. The results suggest that the distinctive features of heat-shock promoters, which underlie the massive and rapid expression of heat-shock genes upon heat shock, also are a source of evolutionary variation on which natural selection can act.
- Subjects
DROSOPHILA melanogaster; HEAT shock proteins; MUTAGENESIS; NUCLEOTIDES; MESSENGER RNA; GENETICS; GENES
- Publication
Genetics, 2006, Vol 173, Issue 2, p809
- ISSN
0016-6731
- Publication type
Article
- DOI
10.1534/genetics.105.053959