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- Title
Expression of pro-angiogenic genes in mesenchymal stem cells derived from dermis of patients with psoriasis.
- Authors
Niu, Xuping; Chang, WenJuan; Liu, Ruifeng; Hou, Ruixia; Li, Junqin; Wang, Chunfang; Li, Xinhua; Zhang, Kaiming
- Abstract
Background Recent experimental studies revealed that angiogenesis and lymphangiogenesis are closely related to psoriasis. Our microarray analysis suggested that the pro-angiogenic genes platelet endothelial cell adhesion molecule-1 ( PECAM1), facio-genital dysplasia-5 ( FGD5), prostaglandin-endoperoxide synthase-1 ( PTGS1), melanoma cell adhesion molecule ( MCAM), vasohibin- 2 ( VASH2), and stabilin-1 ( STAB1) are differentially expressed in dermal mesenchymal stem cells in psoriasis. Objectives The aim of this study was to investigate the mRNA and protein expression of PECAM1, FGD5, PTGS1, MCAM, VASH2, and STAB1 for angiogenesis and the possible mechanisms in psoriasis. Methods We studied 12 patients with plaque psoriasis and 14 healthy controls matched for age and sex. Dermal mesenchymal stem cells were expanded, passaged, and identified by cellular morphology, immunophenotyping, and multipotential differentiation. The mRNA and protein expression of the above-mentioned six genes were confirmed by quantitative real-time reverse transcription-polymerase chain reaction and Western blotting. Results The significantly decreased expression of PECAM1, PTGS1, FGD5, and MCAM at both mRNA and protein level (except VASH2 and STAB1) were demonstrated in mesenchymal stem cells from psoriatic skin lesions compared with non-lesional from healthy controls. Conclusions We provide the first report that pro-angiogenic genes PECAM1, PTGS1, FGD5, and MCAM rather than VASH2 and STAB1 may be play a vital role in pathological dermal angiogenesis disorders of psoriasis. Therefore, anti-angiogenesis is attractive and offers future potential for application in patients with psoriasis.
- Subjects
MESSENGER RNA; GENE expression; NEOVASCULARIZATION; PSORIASIS; MESENCHYMAL stem cells; CELL morphology; CELL differentiation
- Publication
International Journal of Dermatology, 2016, Vol 55, Issue 5, pe280
- ISSN
0011-9059
- Publication type
Article
- DOI
10.1111/ijd.13197