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- Title
Molecular and morphological characterisation of Laimaphelenchus spiflatus n. sp. (Nematoda: Aphelenchoididae) from China.
- Authors
Gu, Jianfeng; Maria, Munawar; Fang, Yiwu; Chen, Xianfeng; Liu, Lele
- Abstract
Summary: Laimaphelenchus spiflatus n. sp. isolated from declining Chinese pine, Pinus tabuliformis , is described and illustrated. The new species can be characterised by its relatively long body size of 1150 ± 108 (976-1437) μ m for females and 1092 ± 78.6 (905-1235) μ m for males, lateral field with four lines, females with a long vulval flap, and tail conoid, slightly ventrally curved and ending in a stalk having 8-12 projections when observed with SEM. Males are common, with 27.3 (23.4-28.8) μ m long spicules having blunt (not well-developed) condylus and rostrum and truncate simple distal tip, and four caudal papillae. By having a vulval flap, four lateral lines and tail end with a stalk (without four tubercles), the new species comes close to L. preissii , L. simlaensis , and L. unituberculus , but can be differentiated from them by morphological characters and morphometric data. In phylogenetic analyses using near full length 18S and D2-D3 expansion segments of 28S rDNA, Laimaphelenchus spiflatus n. sp. appeared as an independent lineage separated from the other Laimaphelenchus spp. that are currently sequenced for their aforementioned genomic regions.
- Subjects
CHINA; NEMATODES; BODY size; BEAKS; PINE; RECOMBINANT DNA
- Publication
Nematology, 2020, Vol 22, Issue 8, p843
- ISSN
1388-5545
- Publication type
Article
- DOI
10.1163/15685411-00003344