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- Title
Huntington disease oligodendrocyte maturation deficits revealed by single-nucleus RNAseq are rescued by thiamine-biotin supplementation.
- Authors
Lim, Ryan G.; Al-Dalahmah, Osama; Wu, Jie; Gold, Maxwell P.; Reidling, Jack C.; Tang, Guomei; Adam, Miriam; Dansu, David K.; Park, Hye-Jin; Casaccia, Patrizia; Miramontes, Ricardo; Reyes-Ortiz, Andrea M.; Lau, Alice; Hickman, Richard A.; Khan, Fatima; Paryani, Fahad; Tang, Alice; Ofori, Kenneth; Miyoshi, Emily; Michael, Neethu
- Abstract
The complexity of affected brain regions and cell types is a challenge for Huntington's disease (HD) treatment. Here we use single nucleus RNA sequencing to investigate molecular pathology in the cortex and striatum from R6/2 mice and human HD post-mortem tissue. We identify cell type-specific and -agnostic signatures suggesting oligodendrocytes (OLs) and oligodendrocyte precursors (OPCs) are arrested in intermediate maturation states. OL-lineage regulators OLIG1 and OLIG2 are negatively correlated with CAG length in human OPCs, and ATACseq analysis of HD mouse NeuN-negative cells shows decreased accessibility regulated by OL maturation genes. The data implicates glucose and lipid metabolism in abnormal cell maturation and identify PRKCE and Thiamine Pyrophosphokinase 1 (TPK1) as central genes. Thiamine/biotin treatment of R6/1 HD mice to compensate for TPK1 dysregulation restores OL maturation and rescues neuronal pathology. Our insights into HD OL pathology spans multiple brain regions and link OL maturation deficits to abnormal thiamine metabolism. Here the authors evaluate single cell gene expression from mouse and human Huntington's disease brains, finding incomplete oligodendrocyte maturation and pathways involved. Treating mice with thiamine/biotin ameliorates molecular pathology.
- Subjects
HUNTINGTON disease; RNA sequencing; VITAMIN B1; DIETARY supplements; BRAIN diseases; LIPID metabolism; POSTMORTEM changes; MOLECULAR pathology
- Publication
Nature Communications, 2022, Vol 13, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-022-35388-x