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- Title
Upregulation of a functional form of the β<sub>4</sub> integrin subunit in colorectal cancers correlates with c-Myc expression.
- Authors
Hehong Ni; Dydensborg, Anders Bondo; Herring, Florence Elizabeth; Basora, Nuria; Gagné, David; Vachon, Pierre H.; Beaulieu, Jean-François
- Abstract
The integrin β4 subunit has been shown to be involved in various aspects of cancer progression. The aim of the present work was to evaluate the expression of β4 in primary colon cancers and to investigate the occurrence of a previously identified intestinal nonfunctional variant of β4 (β4ctd−) for adhesion to laminin. Immunodetection of β4 using a panel of antibodies and RT–PCR analyses were performed on series of paired primary colon tumors and corresponding resection margins. The β4 subunit was found to be significantly overexpressed in cancer specimens at both the protein and transcript levels. Surprisingly, β4 levels of expression were closely correlated with those of the oncogene c-Myc in individual specimens. In vitro studies of c-Myc overexpression showed an upregulation of β4 promoter activity. Finally, the β4ctd− form was identified in the normal proliferative colonic cells but was found to be predominantly absent in colon cancer cells, both in situ and in vitro. We concluded that the β4ctd− form is lost from colon cancer cells, while the level of the wild-type form of β4, which is functional for adhesion to laminin, is increased in primary tumors in relation with the expression of c-Myc.Oncogene (2005) 24, 6820–6829. doi:10.1038/sj.onc.1208848; published online 27 June 2005
- Subjects
COLON cancer; INTESTINAL diseases; IMMUNOGLOBULINS; SURGICAL excision; MYC oncogenes; CANCER cells
- Publication
Oncogene, 2005, Vol 24, Issue 45, p6820
- ISSN
0950-9232
- Publication type
Article
- DOI
10.1038/sj.onc.1208848