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- Title
Weak Value Amplification-Based Biochip for Highly Sensitive Detection and Identification of Breast Cancer Exosomes.
- Authors
Zhao, Jingru; Guan, Xiaotian; Zhang, Sihao; Sha, Zhou; Sun, Shuqing
- Abstract
Exosomes constitute an emerging biomarker for cancer diagnosis because they carry multiple proteins that reflect the origins of the parent cell. The highly sensitive detection of exosomes is a crucial prerequisite for the diagnosis of cancer. In this study, we report an exosome detection system based on quantum weak value amplification (WVA). The WVA detection system consists of a reflection detection light path and a Zr-ionized biochip. Zr-ionized biochips effectively capture exosomes through the specific interaction between zirconium dioxide and the phosphate groups on the lipid bilayer of exosomes. Aptamer-modified gold nanoparticles (Au NPs) are then used to specifically recognize proteins on exosomes to enhance the detection signal. The sensitivity and resolution of the detection system are 2944.07 nm/RIU and 1.22 × 10−5 RIU, respectively. The concentration of exosomes can be directly quantified by the WVA system, ranging from 105–107 particles/mL with the detection limit of 3 × 104 particles/mL. The use of Au NPs-EpCAM for the specific enhancement of breast cancer MDA-MB-231 exosomes is demonstrated. The results indicate that the WVA detection system can be a promising candidate for the detection of exosomes as tumor markers.
- Subjects
EXOSOMES; BREAST cancer; ZIRCONIUM oxide; TUMOR markers; SIGNAL detection; BILAYER lipid membranes
- Publication
Biosensors (2079-6374), 2024, Vol 14, Issue 4, p198
- ISSN
2079-6374
- Publication type
Article
- DOI
10.3390/bios14040198