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- Title
CYP24A1 and TRPC3 Gene Expression in Kidneys and Their Involvement in Calcium and Phosphate Metabolism in Laying Hens.
- Authors
Salmória, Letícia Alves; Ibelli, Adriana Mércia Guaratini; Tavernari, Fernando Castro; Peixoto, Jane Oliveira; Morés, Marcos Antônio Zanella; Marcelino, Débora Ester Petry; Pinto, Karine Daenquele Silva; Coldebella, Arlei; Surek, Diego; Kawski, Vicky Lilge; Ledur, Mônica Corrêa
- Abstract
Simple Summary: Calcium and phosphorus are essential minerals required for animal health, especially in laying hens since they are important components in bone and eggshell formation. Diets with inadequate calcium and phosphorus can interfere with their absorption and digestibility, resulting in eggshell quality losses and reduced productive life, affecting egg production and welfare. Although mineral metabolism has been widely studied in young laying hens, the mechanisms of absorption, reabsorption, and mineral homeostasis of laying hens with advancing age are not well known. Therefore, we studied eight genes involved in Ca and P metabolism and observed that two of them (CYP24A1 and TRPC3) showed differential expression according to the Ca/P ratios provided in the diet. These results contribute to understanding how Ca and P metabolism are regulated in hens during their late laying cycle, allowing the development of new nutritional strategies to improve hens' productive life as well as their egg quality. Ca and P homeostasis across the egg-laying cycle is a complex process involving absorption in the small intestine, reabsorption/excretion in the kidneys, and eggshell gland secretion. Diets with inadequate calcium and phosphorus can interfere with their absorption and digestibility, resulting in eggshell quality losses and reduced productive life, affecting egg production and welfare. A better understanding of gene expression profiles in the kidneys of laying hens during the late egg-laying period could clarify the renal role in mineral metabolism at this late stage. Therefore, the performance, egg quality and bone integrity-related traits, and expression profiles of kidney candidate genes were evaluated in 73-week-old laying hens receiving different Ca and P ratios in their diet: a high Ca/P ratio (HR, 22.43), a low ratio (LR, 6.71), and a medium ratio (MR, 11.43). The laying hens receiving the HR diet had improved egg production and eggshell quality traits compared to the other two groups. Humerus length was shorter in the HR than in the other groups. The CYP24A1 and TRPC3 genes were differentially expressed (p.adj ≤ 0.05) among the groups. Therefore, their expression profiles could be involved in calcium and phosphate transcellular transport in 73-week-old laying hens as a way to keep mineral absorption at adequate levels.
- Subjects
EGGSHELLS; HENS; CALCIUM metabolism; PHOSPHATE metabolism; CALCIUM phosphate; GENE expression
- Publication
Animals (2076-2615), 2024, Vol 14, Issue 10, p1407
- ISSN
2076-2615
- Publication type
Article
- DOI
10.3390/ani14101407