We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Induction of Autophagy by Vasicinone Protects Neural Cells from Mitochondrial Dysfunction and Attenuates Paraquat-Mediated Parkinson's Disease Associated α-Synuclein Levels.
- Authors
Huang, Chih-Yang; Sivalingam, Kalaiselvi; Shibu, Marthandam Asokan; Liao, Po-Hsiang; Ho, Tsung-Jung; Kuo, Wei-Wen; Chen, Ray-Jade; Day, Cecilia-Hsuan; Viswanadha, Vijaya Padma; Ju, Da-Tong
- Abstract
Mitochondrial dysfunction and disturbed mitochondrial dynamics were found to be common phenomena in the pathogenesis of Parkinson's disease (PD). Vasicinone is a quinazoline alkaloid from Adhatoda vasica. Here, we investigated the autophagy/mitophagy-enhancing effect of vasicinone and explored its neuroprotective mechanism in paraquat-mimic PD modal in SH-SY5Y cells. Vasicinone rescued the paraquat-induced loss of cell viability and mitochondrial membrane potential. Subsequently, the accumulation of mitochondrial reactive oxygen species (ROS) was balanced by an increase in the expression of antioxidant enzymes. Furthermore, vasicinone restored paraquat-impaired autophagy and mitophagy regulators DJ-1, PINK-1 and Parkin in SH-SY5Y cells. The vasicinone mediated autophagy pathways were abrogated by treatment with the autophagy inhibitor 3-MA, which lead to increases α-synuclein accumulation and decreased the expression of p-ULK and ATG proteins and the autophagy marker LC3-II compared to that observed without 3-MA treatment. These results demonstrated that vasicinone exerted neuroprotective effects by upregulating autophagy and PINK-1/Parkin mediated mitophagy in SH-SY5Y cells.
- Subjects
DRUG therapy for Parkinson's disease; AUTOPHAGY; REACTIVE oxygen species; ALKALOIDS; ANTIOXIDANTS; ANTIPARKINSONIAN agents; CELL lines; CELLULAR signal transduction; GENE expression; MITOCHONDRIA; NERVE tissue proteins; NEURONS; OXIDOREDUCTASES; NEUROPROTECTIVE agents; CELL survival; MEMBRANE transport proteins; PHARMACODYNAMICS
- Publication
Nutrients, 2020, Vol 12, Issue 6, p1707
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu12061707