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- Title
Regressive Effect of Myricetin on Hepatic Steatosis in Mice Fed a High-Fat Diet.
- Authors
Shu-Fang Xia; Guo-Wei Le; Peng Wang; Yu-Yu Qiu; Yu-Yu Jiang; Xue Tang
- Abstract
Myricetin is an effective antioxidant in the treatment of obesity and obesity-related metabolic disorders. The objective of this study was to explore the regressive effect of myricetin on pre-existing hepatic steatosis induced by high-fat diet (HFD). C57BL/6 mice were fed either a standard diet or a HFD for 12 weeks and then half of the mice were treated with myricetin (0.12% in the diet, w/w) while on their respective diets for further 12 weeks. Myricetin treatment significantly alleviated HFD-induced steatosis, decreased hepatic lipid accumulation and thiobarbituric acid reactive substance (TBARS) levels, and increased antioxidative enzyme activities, including catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities. Microarray analysis of hepatic gene expression profiles showed that myricetin significantly altered the expression profiles of 177 genes which were involved in 12 biological pathways, including the peroxisome proliferator activated receptor (PPAR) signaling pathway and peroxisome. Further research indicated that myricetin elevated hepatic nuclear Nrf2 translocation, increased the protein expression of heme oxygenase-1 (HO-1) and NAD(P)H quinone dehydrogenase 1 (NQO1), reduced the protein expression of PPAR, and normalized the expressions of genes that were involved in peroxisome and the PPAR signaling pathway. Our data indicated that myricetin might represent an effective therapeutic agent to treat HFD-induced hepatic steatosis via activating the Nrf2 pathway and the PPAR signaling pathway.
- Subjects
CHINA; THERAPEUTIC use of antioxidants; FATTY liver prevention; ANIMAL experimentation; BIOMARKERS; HIGH performance liquid chromatography; INGESTION; MICE; POLYMERASE chain reaction; RESEARCH funding; STATISTICAL sampling; STATISTICS; T-test (Statistics); DATA analysis; OXIDATIVE stress; REVERSE transcriptase polymerase chain reaction; DATA analysis software; OLIGONUCLEOTIDE arrays; SIGNAL peptides; DESCRIPTIVE statistics; IN vitro studies; ONE-way analysis of variance; IN vivo studies
- Publication
Nutrients, 2016, Vol 8, Issue 12, p799
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu8120799