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- Title
Host-induced silencing of a nematode chitin synthase gene decreases abundance of rhizosphere fungal community while enhancing Heterodera glycines resistance of soybean.
- Authors
Tian, Shuan; Shi, Xue; Qu, Baoyuan; Kang, Houxiang; Huang, Wenkun; Peng, Huan; Peng, Deliang; Wang, Jiajun; Liu, Shiming; Kong, Lingan
- Abstract
Context: A transgenic variety of soybean (Glycine max (L.) Merr.), H57, has been developed from wild-type variety Jack, with host-induced gene silencing of a chitin synthase gene (CHS) in soybean cyst nematode (SCN, Heterodera glycines Ichinohe), a devastating pathogen in soybean. H57 needs to be characterised for suitability to manage SCN, especially because rhizosphere microbial communities may be sensitive to genetically modified crops. Aims: We aimed to evaluate the SCN resistance of H57 at the T7 generation, and analyse the impact on the rhizosphere microbial community of planting H57 into SCN-infected soil. Methods: Infection with SCN was assessed at 60 days after planting of H57 and Jack into SCN-infected soil by examining recovered cysts from rhizosphere soil and comparing with an infected bulk soil control. For analysis of rhizosphere microbial communities (bacterial and fungal), 16S and ITS amplicons were identified by high-throughput sequencing, and bioinformatic analysis was used to define operational taxonomic units. Alpha diversity, using five indexes, and relative abundance were determined. Key results: Soybean H57 showed significantly enhanced and heritable resistance to SCN compared with Jack. The diversity and richness (abundance) of the bacterial community of H57 and Jack were significantly and similarly increased relative to the bulk soil. The fungal community of H57 had considerably lower abundance than both other treatments, and lower diversity than the bulk soil. The relative abundance of only two bacterial phyla (Acidobacteria and Actinobacteria) and one fungal phylum (Glomeromycota), and three bacterial genera (Candidatus _Solibacter, Candidatus _Udaeobacter and Bryobacter) and one fungal genus (Aspergillus), differed significantly between rhizosphere soils of H57 and Jack. Conclusions: Host-induced gene silencing of SCN-CHS substantially and heritably enhanced SCN resistance in soybean, did not significantly alter the rhizosphere bacterial community, but greatly suppressed the abundance of the rhizosphere fungal community, which was likely associated with boosted SCN resistance. Implications: This study established a basis for interaction research between soybean with SCN-CHS host-induced gene silencing and the rhizosphere microbial community, and for potentially planting soybean H57 to manage SCN. A T7 generation host-induced gene silencing (HIGS) transgenic soybean variety, H57, targeting a soybean cyst nematode chitin synthase gene (SCN-CHS) showed heritable enhanced SCN resistance. HIGS of SCN-CHS substantially suppressed abundance of rhizosphere fungal community, likely associated with enhanced SCN resistance of H57. This study provides a basis for interaction research between SCN-CHS HIGS soybean and the rhizosphere microbial community and for potential use of soybean H57 to manage SCN.
- Subjects
SOYBEAN cyst nematode; CHITIN synthase; FUNGAL communities; RHIZOSPHERE; TRANSGENIC plants; GENE silencing; SOYBEAN
- Publication
Crop & Pasture Science, 2022, Vol 73, Issue 10, p1156
- ISSN
1836-0947
- Publication type
Article
- DOI
10.1071/CP22030