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- Title
The effects of biodegradable poly(lactic‐co‐glycolic acid)‐based microspheres loaded with quercetin on stemness, viability and osteogenic differentiation potential of stem cell spheroids.
- Authors
Lee, H.; Nguyen, T. T.; Kim, M.; Jeong, J.‐H.; Park, J.‐B.
- Abstract
Objective: Quercetin has been reported to exert many beneficial effects on the protection against various diseases, such as diabetes, cancer, and inflammation. The aim of this study is to evaluate the potential osteogenic differentiation ability of mesenchymal stem cells in the presence of quercetin. Material and Methods: Quercetin‐loaded poly(lactic‐co‐glycolic acid) microspheres were prepared using an electrospraying technique. Characterization of the microspheres was evaluated with a scanning electron microscope and release profile. Three‐dimensional cell spheroids were fabricated using silicon elastomer‐based concave microwells. Qualitative results of cellular viability were seen under a confocal microscope, and quantitative cellular viability was evaluated using the Cell Counting Kit‐8 assay. The alkaline phosphatase activity and Alizarin Red S staining were performed. A quantitative real‐time polymerase chain reaction and a western blot analysis were performed. Results: Spheroids were well formed irrespective of quercetin concentration. Most of the cells in spheroids emitted green fluorescence, and the morphology was round without significant changes. The application of quercetin‐loaded microspheres produced a significant increase in the alkaline phosphatase activity. The real‐time polymerase chain reaction results showed a significant increase in Runx2, and western blot results showed higher expression of Runx2 protein expression. Conclusion: Biodegradable microspheres loaded with quercetin produced prolonged release profiles with increased mineralization. Microspheres loaded with quercetin can be used for the enhancement of osteoblastic differentiation in cell therapy.
- Subjects
POLYLACTIC acid; GLYCOLIC acid; QUERCETIN; MESENCHYMAL stem cell differentiation; MICROSPHERES; BIODEGRADABLE nanoparticles; RUNX protein genetics; ALKALINE phosphatase; BIOLOGICAL assay; CELL differentiation; CELLULAR therapy; ELASTOMERS; GENE expression; LATEX; POLYESTERS; POLYMERASE chain reaction; SCANNING electron microscopy; SILICON; STAINS &; staining (Microscopy); STEM cells; WESTERN immunoblotting
- Publication
Journal of Periodontal Research, 2018, Vol 53, Issue 5, p801
- ISSN
0022-3484
- Publication type
Article
- DOI
10.1111/jre.12569