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- Title
Pingchuanning Decotion Alleviates Bronchial Asthma Airway Inflammation Through ROS/ HMGB1/Beclin-1 Mediated Cell Autophagy.
- Authors
Xinheng Wang; Yating Gao; Lingxiu Li; Qinjun Yang; Lu Zhang; Weidong Ye; Xiangming Fang; Zegeng Li
- Abstract
Objective • Bronchial asthma is a prevalent respiratory disorder characterized by airway inflammation. This study aimed to investigate the protective effect of Pingchuanning decoction (PCN) on airway inflammation in bronchial asthma, focusing on the role of autophagy and its underlying molecular mechanism. Methods • Using an in vitro lipopolysaccharide (LPS)- induced inflammatory damage model of human airway epithelial cells (16HBE), we assessed the effect of PCN. Various experiments were performed to evaluate the expression of autophagy-related genes, autophagosome and vesicle counts, and reactive oxygen species (ROS) levels. Results • First, PCN reduced LPS-induced cellular inflammation. Second, PCN decreased the number of autophagosomes and autophagic vesicles. And third, PCN significantly reduced reactive oxygen species (ROS) levels. Most importantly, PCN also down-regulated LPS-induced expression of HMGB1, Beclin-1, and autophagy-related gene 5 (ATG5) while enhancing the expression of B-cell lymphoma 2 (Bcl-2), which further reduced the LC3II/I ratio. Conclusion • PCN reduces the 16HBE inflammatory response by inhibiting the overexpression of ROS/ HMGB1/Beclin-1 mediated cell autophagy. Therefore, it may serve as a potential drug for treating bronchial asthma.
- Subjects
ASTHMA treatment; CHINESE medicine; LIPOPOLYSACCHARIDES; INFLAMMATION treatment
- Publication
Alternative Therapies in Health & Medicine, 2024, Vol 30, Issue 1, p270
- ISSN
1078-6791
- Publication type
Article