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- Title
Identification and validation of single nucleotide polymorphisms as tools to detect hybridization and population structure in freshwater stingrays.
- Authors
Cruz, Vanessa P.; Vera, Manuel; Pardo, Belén G.; Taggart, John; Martinez, Paulino; Oliveira, Claudio; Foresti, Fausto
- Abstract
Single nucleotide polymorphism ( SNP) markers were identified and validated for two stingrays species, Potamotrygon motoro and Potamotrygon falkneri, using double digest restriction-site associated DNA (ddRAD) reads using 454-Roche technology. A total of 226 774 reads (65.5 Mb) were obtained (mean read length 289 ± 183 bp) detecting a total of 5399 contigs (mean contig length: 396 ± 91 bp). Mining this data set, a panel of 143 in silico SNPs was selected. Eighty-two of these SNPs were successfully validated and 61 were polymorphic: 14 in P. falkneri, 21 in P. motoro, 3 in both species and 26 fixed for alternative variants in both species, thus being useful for population analyses and hybrid detection.
- Subjects
SINGLE nucleotide polymorphisms; SPECIES hybridization; FRESHWATER stingrays; POTAMOTRYGON; DNA restriction enzymes
- Publication
Molecular Ecology Resources, 2017, Vol 17, Issue 3, p550
- ISSN
1755-098X
- Publication type
Article
- DOI
10.1111/1755-0998.12564